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Briefly, MODE-K IECs were silenced for 48 h with siGpx4 or siCtrl and washed with high-glucose DMEM (1% FCS, 1% penicillin/streptomycin, 25 mM HEPES)

From a formulation standpoint, this mechanism explains why we consistently observe greater stability in finished SAG products than in GSH: GSH oxidizes before it reaches the cell, whereas SAG arrives intact and activates on demand