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Despite RIC is classified as an autosomal recessive disorder, five of the seven Japanese patients with RIC in a retrospective study were compound heterozygous, and two were simple heterozygous with a single variant of the ATP8B1 or ABCB11 genes

Previous observations also highlighted the stimulatory role of IGF-2 in the proliferation [18] and expression of progesterone and estradiol in luteinized granulosa cells in humans [19, 20]

Maisch, Germany, Ammerbuch-Entringen) analytical column (prepared in-house) at a constant pressure of 925 bar (flow rate of circa 700 nL/min) with 1% formic acid in water and eluted at a flow of 0.5 L min 1 with a 50 min linear gradient from 9% to 34% acetonitrile in water and 1% formic acid with a Thermo Vanquish Neo nanoLC (Thermo Fisher, Waltham, MA)

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