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Louis, MO, USA) at a concentration of 7 mg/ml (50% ACN with 0.1% TFA) was sprayed over the tissue sections using the TM-Sprayer with the following settings: 100 l/min, 10 passes, crisscross pattern, a velocity of 1,300 mm/min, 3 mm track spacing

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Studies suggest that dysbiosis may affect the metabolism of dietary components, increase inflammation, and alter gut hormone signaling, leading to the development of obesity

Certain beneficial gut microbes, such as Lactobacillus and Bifidobacterium , have been shown to stimulate the vagus nerve, leading to anxiolytic and antidepressant-like effects through the modulation of neurotransmitter release and HPA axis regulation [124, 150]