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The study also reported changes in the signaling-related proteins c-Fos, c-Jun, and Egr-1

After 5 min of pre-incubation at 37 C, 10 L of 20 mM nicotinamide adenine dinucleotide phosphate (NADPH) tetrasodium salt in PBS was added as cofactor to initiate the reaction, followed by incubation for a further 60 min at a shaking frequency of 550 rpm