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All efforts were made to minimize animal suffering and the number of animals used

If an athlete is interested in trying supplements in category II (i.e., Limited or Mixed Evidence to Support Efficacy), the athlete should make sure they understand these supplements are more experimental and they may or may not see the type of results claimed

Mild fatigue afterward is common, and hydration and rest are encouraged

Nevertheless, although a specific action of homocysteine seems to occur at low concentrations, experimental trials have been conducted only with homocysteine administration, which would reflect a severe hyperhomocysteinemia rather than a more physiologic moderate elevation of circulating homocysteine, and clinical observations have only come from homocystinuric patients with severe hyperhomocysteinemia