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20 L of each serial dilution was added to 180 L of blood on ice for final concentrations of 0.01 g/mL, 0.1 g/mL, 1 g/mL and 10 g/mL

The uptake reaction was stopped by adding ice-cold phloretin (Sigma-Aldrich) to a final concentration of 200 M and centrifugation through an oil layer (1:1 Dow Corning 550 Silicon fluid (Motion Industries, Birmingham, AL) and dinonyl phthalate (Sigma-Aldrich))

With over five years of nursing experience in emergency medicine and correctional healthcare, Sadie brings a compassionate, patient-centered approach to wellness and functional health

Bacteriostatic Water is a sterile water solution containing 0.9% benzyl alcohol , which functions as a preservative to inhibit bacterial growth