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FIGURE 4 3.4 Imaging GSH in living pulmonary cells Before the intracellular imaging procedure, the cytotoxicity of the probe DSNBD-GSH on living pulmonary cells (A549 and BEAS-2B cell lines) was investigated with the MTT assay

GPx activity GPx activity was measured according to the method of Inoue et al

The metabolism of starch and sucrose (15 KOs), was also reduced in the AcM groups ( PYG , sucrose phosphorylase , GBE1 , glgA , UGP2 , glgC , malZ , INV , 6-phospho-beta-glucosidase , pgmB , celA , celB , scrA , bglX and inuJ ), together with the depletion of the phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS), and with the biosynthesis of cofactors (31 KOs), including those involved in the metabolism of vitamins, such as thiamine ( adk , thiD , thiI and iscS ), riboflavin ( ribF ), pantothenate ( coaA , coaBC and coaE ), pyridoxine ( pdxK ) and folate ( DHFR , folC , folD , fhs , glyA and metK )