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residual crosslinker can bias enrichment

Yan T, Ma Z, Liu J, Yin N, Lei S, Zhang X, et al

From these enzymes, glutathione peroxidase, catalase, and superoxide dismutase together form a common system aimed at protecting the cell from peroxidant molecules [38]

42 This results in changes to membrane permeability, collapse of membrane potentials, and ion gradients, ultimately triggering cell and organelle swelling