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EE and RER were calculated based on the oxygen consumption (O 2 ) and carbon dioxide production (CO 2 )

Despite these limitations, FSEC-TS should be considered when absolute T m values are not necessarily required, for example, in the screening of stable homologs or mutants for structural study, or screening of lipids, ligands for purification, owing to its simplicity, general applicability, and high-throughput feature