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New England Journal of Medicine (2022) 4

The BioSpectrum Imaging System (UVP, LLC) was used to detect signal of immunopositive protein bands

Dot blot Commercially available human recombinant pro-BDNF (Alomone Labs) and two types of mature-BDNF (Sigma and Alomone Labs) were spotted on a nitrocellulose membrane at the concentration of 10 pg and 1000 pg per lane, respectively