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The bacteriostatic water provides some protection, but repeated access with imperfect sterile technique introduces cumulative contamination risk

showed an increase in AMPK phosphorylation and SIRT1 expression levels, while in vitro results revealed a reduction in lipid accumulation and an increase in lipolysis

Nevertheless, the worldwide challenges of chitosan in drug nanoparticle technology remain, such as the limitation of the material source, time-consuming manufacture, unreliable production yield, and non-environmentally friendly synthesis due to the requirement of harsh chemical treatment (Kou et al., 2021)