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The animals were then deeply anesthetized with 3% isoflurane and 50 mg/kg of ketamine followed by a laminectomy at lumbar level L2-L3, corresponding to the sacrocaudal spinal cord

The discrepancies between both studies could be due, at least in part, to the different anatomic injection site employed, since, as indicated above, thigh injections have been rated more painful than injections in the abdomen [25, 26]

Preclinical research models have explored its interactions with angiogenic signaling, tissue response pathways, and receptor-associated biological mechanisms in small-animal and in-vitro studies

Usually, these symptoms are mild and go away after a few days