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After washing and quenching, slides were blocked with buffer (1% bovine serum albumin (BSA) and 2.5% normal goat serum in TBS-T) for 20 min

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Relative transcripts levels were calculated using the modified Ct method (Pfaffl, Determination of the expression of the KLIKK proteases in vivo Patients with diagnosed chronic periodontitis attending the Clinic of Periodontology at the University Hospital of Jena were recruited for this study

We thank the University of Chicago Cytometry and Antibody Technology (CAT) Core Facility for flow cytometry instrument support and the Genomics Core Facility for assistance with mRNA microarray analysis