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254 SURFACE functionalization MSNs have very competitive applications in various areas of biomedicine, to a large extent due to their hydrophilic surface containing a large proportion of SiOH groups, which makes them susceptible to various functionalization modifications on the external or internal porous surface

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These methods optionally further involve: (3) reassembling (&/or subjecting to one or more directed evolution methods described herein) at least one optimized recombinant nucleic acid with a further form of the nucleic acid, which is the same or different from the first and second forms, to produce a further library of recombinant nucleic acids