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Glutathione-S-transferase (GST) tag was added at the C-terminus of CTD by PCR amplifying CTD gene from pETGP10CTD using the oligonucleotides listed in Table 2, digesting the amplicon with Eco RI and Bam HI, and ligating it in pGEX-4T-2 vector at the same sites to yield pGEXCTD-GST