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Retrieved February 22, 2012

J Endocrinol 166 : 579590 Coschigano KT et al

For hormone and metabolite analyses, animals were fasted at 0800 h, and trunk blood was routinely collected 4 h later at 1200 h by decapitation without anesthesia, and plasma was separated from red cells by centrifugation (1500 g 4 C 15 min) and stored at 80 C