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Inferring the molecular and phenotypic impact of amino acid variants with MutPred2

Briefly, for each PCR reaction, 50 ng of cDNA were amplified with each primer set using a Bio-Rad iCycler (Bio-Rad Laboratories Ltd., Mississauga, Ontario) and the amplification conditions described by Frutos et al

There are other phospholipids that comprise the cell membrane such as phosphatidylserine and phosphatidylethnalomine, but phosphatidylcholine makes up the majority of the building blocks

It is reported that after administrated with metronidazole (500 mg twice daily) plus ciprofloxacin (500 mg once daily) for 1 week, near-complete suppression of detectable TMAO and d9-TMAO are observed under the challenge of phosphatidylcholine in both plasma and urine (Tang et al., 2013)