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Brand: chapuisstores.ch
SKU: 12431300380
Price: 27.97 USD
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Description

Sticova E, Jirsa M

Cell Immunol (2016) 299:1422.10.1016/j.cellimm.2015.10.006 159 BrandAMVargheseGMajewskiWHawdonJM

For purification, a cell pellet was resuspended in lysis buffer containing 50 mM Tris pH 7.5, 500 mM NaCl, 50 ug/ml DNase1, 50 ug/m lysozyme, 0.5 mM Benzamidine, 0.5 mM PMSF, and EDTA Free protease inhibitor cocktail (Roche), sonicated and debris removed by a 45-min centrifugation at 50,000 x g