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Cells were lysed with 500 L of RIPA lysis buffer containing 150 mM NaCl, 1% NP-40, 0.5% sodium deoxycholate, 0.1% SDS, 25 mM Tris-HCl, supplemented with protease and phosphatase inhibitors at a 1:100 dilution

Among these, receptor-interacting protein 3 (RIP3), phosphorylated mixed lineage kinase domain-like protein (p-MLKL), and ferritin light chain 1 (FTL1) exhibit significant changes in expression and are associated with ferroptosis (Cao et al., 2021)