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Description

The centrifugation process was performed after 10 min at 400g for 10 min, followed by separation and disposal of the plasma and buffy coat

J.NaikP.KapasS

For protein quantification, the remaining 50 L of the cell suspension underwent 3 freeze-thaw cycles using liquid nitrogen, followed by centrifugation at 13,800 g for 10 min

Additionally, by activating caspase 1 and cleaving pro-IL-1 and pro-IL-18 to generate IL-1 and IL-18, respectively, ATM and DNA-PKcs support AIM2 inflammasome activation [146, 147]