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In the present study, the stability of recombinant TAT-CcSOD1 was further detected, and the results showed that TAT-CcSOD1 could retain high activity under a wide range of pH (5.010.0) and stayed stable in temperature 70 C for at least 1 h, exhibiting a distinct thermostability and a wide alkali and acerbic tolerance range, which is similar to that of CcSOD1

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The mobile phase comprised of (A) 10 mM ammonium formate in 60% aqueous acetonitrile containing 0.1% formic acid and (B) 10 mM ammonium formate in 9:1 mixture of isopropanol and acetonitrile with a 0.5 mL/min flow rate maintained during a 20 min run

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