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Once a clear stock solution has been prepared in acetic acid water, it is standard practice to perform subsequent dilutions into the intended experimental vehicle for example, phosphate-buffered saline (PBS), cell culture medium, or another physiological buffer to bring the working solution to the correct pH and ionic strength for the assay

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TOP Vitamin B 12 B 12 is a water-soluble vitamin found in foods derived from animal products and from fortified foods