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In order to gain insights into biological pathways associated with increasing emphysema severity within a patient and explore computational strategies for identifying COPD therapeutics, we obtained paired samples from eight regions at regular intervals between the apex and base of each explanted lung from six patients with severe COPD (Global Initiative for Chronic Obstructive Lung Disease (GOLD) stage IV) and two donor lungs

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Where indicated, HFF-1-containing media were also incubated with a different treatment of either 10 or 30 mM GSH, or 4 g/mL amikacin alone, or a combination of 10 mM GSH + 4 g/mL amikacin, either in the presence or absence of bacteria

Upon excitation with visible light, it can undergo both Type I (radical-mediated) and Type II (singlet oxygen-mediated) photochemical reactions