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In fatty liver disease specifically, elevated B12 would more likely occur in advanced stages with significant liver damage rather than in simple steatosis

Samples were then evaporated to dryness in Speed vac and resuspended in 500 l Buffer A (95% H 2 O, 5% CH 3 CN, 0.1% FA) with sonication and loaded on HPLC for offline high pH fractionation following conditions adapted from previous work 57

An aberrant gene gets turned on during the early stages of fetal development, altering the body