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GSH may become compromised by oxidative stress induced by several factors in the course of serious infections, such as oxidative bursts by activated macrophages, hypergeneration of superoxide, TNFa-generated oxidative stress, induction of Cox-2 with increased arachidonate oxidations, and induction of iNOS with increased formation of nitric oxides and nitrosated GSH

RNA purity and integrity were determined using a NanoDrop ND-1000 (NanoDrop Technologies) and an Agilent 2100 Bioanalyzer (Agilent Technologies)