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The same amount of protein (400 g) were used for all samples, and 8 M Urea lysis buffer was used as a diluent if required

Consult a healthcare provider before supplementing if you: Take blood thinners (vitamin D affects clotting) Use metformin, or proton pump inhibitors (both affect B12 absorption) Have kidney disease (impairs vitamin D metabolism) Have a history of kidney stones (excess vitamin D increases risk) Are being treated for any chronic condition Testing first ensures you get the right dose

treatment should be discontinued immediately if pregnancy is suspected or confirmed