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Lysates were combined with 6 Laemmli SDS sample buffer (Boston BioProducts, BP-111R) containing 5% 2-mercaptoethanol (VWR Life Science, M131), then loaded onto 420% Criterion TGX pre-cast gels (Bio-Rad, 5671093)

308 However, these treatments can cause side effects, such as systemic toxicity, unavoidable invasiveness, and a high risk of relapse

Behavioral observations were performed regularly

This finding is particularly encouraging for other groups who work on resolving these biases or understanding the changes in regional circulation patterns under different climate scenarios