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In contrast, ALA exerts its principal effects on mitochondrial metabolism through its established role as an essential cofactor for mitochondrial -ketoacid dehydrogenase complexes, including pyruvate dehydrogenase and -ketoglutarate dehydrogenase, thereby facilitating efficient entry of glycolytic substrates into the tricarboxylic acid cycle and sustaining electron transport chain activity under oxidative stress conditions

Given the broad and orthogonal substrate scopes established for Cp1B and Cp1D, a library of more than 1,200 E-64 analogs can be generated in one-pot reactions

In this model, genes associated with the one-carbon metabolic pathways, including the folate and methionine cycles, are up-regulated in the fungal cells upon infection, and are supposed to result in enhanced biosynthesis of nucleotides, NADPH, glutathione and methyl groups

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