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Briefly, the slides were incubated with 3% H 2 O 2 to eliminate endogenous peroxidase activity and then blocked with 5% BSA followed first by incubation with primary antibodies against VEGFA (1:200) and anti-IL8 (1:200) overnight at 4 C, and second by incubation with a secondary antibody

It is quite tough to diagnose Vitamin B12 deficiency because its symptoms are not shown

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