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T cells were cultured in human T cell medium (HTCM) consisting of X-Vivo 15 (Lonza, 04-418Q), 5% human AB serum (Fisher Scientific, NC9310328) and 10 mM neutralized N -acetyl-l-cysteine (Sigma-Aldrich, A9165) supplemented with 30 U per ml IL-2 (PeproTech, 200-02 or BRB preclinical biologics repository, National Institutes of Health (NIH)) at 37 C under 5% CO 2

In this kind of apoptosis, Bax activation and translocation leads to status alterations of mitochondrial permeability transition pores, facilitating depolarization of mitochondrial membrane potential and subsequent release of cytochrome c and other apoptosis-promoting proteins to cytosol (Kim et al., 2021

Reznick AZ, Packer L (1994) Oxidative damage to proteins: spectrophotometric method for carbonyl assay