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( f ) HCC1428 cells infected with a control shRNA (Co.) or 3 shRNAs targeting EZH2 (#1, #2, #3) were subjected to immunoblotting with the indicated antibodies

Given that Glu is primarily neuroactive while Gln is storable and neuroinactive, the Gln/Glu ratio can reflect the efficiency of Glu uptake by astrocytes and its conversion to Gln, which is crucial for regulating excitatory neurotransmission [20]