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Required materials: Lyophilized CJC-1295 vial (from a qualified supplier with current COA) Reconstitution solvent (bacteriostatic water or sterile water for injection) Sterile disposable syringe (1 mL or 3 mL, depending on volume) Sterile disposable needle (appropriate gauge for your vial septum) Alcohol swabs (70% isopropyl alcohol) COA confirming fill weight Calculator or concentration calculation sheet Labeling materials (waterproof marker, labels with date, compound, concentration, lot) Optional: laminar flow hood, disposable gloves, lab coat Reference information needed: Vial fill weight (in milligrams) from the supplier COA Target working concentration for your protocol (e.g., 1 mg/mL, 2 mg/mL) Step 1: Verify the Vial Contents and COA Before touching the vial, confirm you have the correct compound and lot

doi: 10.1186/s12993-015-0064-x 142 ChenZYangJZhongJLuoYDuWHuCet al