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However, this method has two limitations: high H 2 O 2 concentrations can inhibit enzyme activity, and UV light can be absorbed by proteins and DNA, making it unsuitable for samples containing these materials (Mukhtar et al

Since it is water-soluble, the body typically eliminates it through urine

Another pathway is the alternative pathway, also named the acidic pathway (25%)

Organellar crosstalk underpins NETosis execution: endoplasmic reticulum (ER)-derived Ca 2+ mobilizes NADPH oxidase complexes, while mitochondrial ROS amplifies oxidative signaling (Zhu et al., 2022)