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Using a novel and validated fluorescent KRAS Y137W variant amenable to stopped-flow spectroscopy, we characterized the kinetics of KRAS G12C fluorescence changes upon addition of ARS-853 or AMG 510, noting that ARS-853 addition at 5C elicited both a rapid first phase (attributed to binding, yielding a K d of 36.0 0.7 M), and a second, slower pH-dependent phase taken to represent covalent ligation