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In the next experiment, buthionine sulfoximine (BSO, 10mM), the inhibitor of gammaglutamylcysteine synthetase was added during 24 hours to A549 cell culture (Figure 4)

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The two compounds activate distinct somatotroph receptors (GHRH-R and GHS-R1a), producing additive GH release in published rodent and primate models without the cortisol or prolactin spillover seen with earlier GHRPs, because they couple to different intracellular signalling cascades