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Angiogenic 3-Dimensional Co-Culture Assay HUVEC (Lonza) cells were transfected with lentiviral GFP and human brain derived pericytes (Sciencell) were transfected with lentiviral Red Fluorescent Protein (dsRED), according to the manufacturers instructions (Dharmacon)

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Local TH activation is crucial for UCP1 expression and consequently adaptive thermogenesis, and its impairment despite normal serum TH levels could suggest reduced thermogenic capacity or function under prolonged stress [29, 30]