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2.5 Hematological analyses After 8 weeks of administration, the remaining 40 rats were anesthetized with isoflurane, then blood was collected in a heparin sodium tube, and the left hind limb was amputated at 1 cm above the ankle joint

A striking increase in oxidized GSH (GSSG increased from 5% at baseline to about 40% 3 h after treatment) was noted, possibly reflecting the potential value of GSH as an antioxidant providing protection in the lung tissue

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