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From a standpoint of inflammation, the activation of the nucleotide-binding oligomerization domain, leucine-rich repeat and pyrin domain-containing protein 3 (NLRP3) inflammasome pathway has proposed the possibility of cuproptosis to interact with pyroptosis, which is an inflammatory form of lytic programmed cell death

Later, as aerobic metabolisms evolved, ROS became essential for signalling

To induce EAU, 30 g of IRBP peptide in a volume of 100 l was mixed with an equal volume of complete Freund's adjuvant (CFA) containing 2.5 mg/ml Mycobacterium tuberculosis strain 37RA