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Standard ice packs are insufficient

Gouty D, Cai CC, Cai XY, Kasinath A, Kumar V, Alvandkouhi S, et al

The cells were collected by centrifugation at 2000 g for 5 min and washed in phosphate buffered saline (8 gL 1 NaCl, 0.2 gL 1 KCl, 1.44 gL 1 Na 2 PO 4 , 0.24 g L 1 KH 2 PO 4 , at pH 7.5)