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The stomach was then exposed by laparotomy, quickly ligated at the pylorus and the cardia and removed

Briefly, reactions were incubated initially at 95 C for 60 s followed by 40 to 45 cycles of 95 C (15 s), 60 C (30 s), and 60 to 95 C on a Roche Lightcycler 480 (Roche Diagnostics, Rotkreuz, Switzerland)