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Following a 72 h dialysis period, the solution was freeze-dried by using low-temperature freeze dryers, resulting in P2 (287 mg), which appeared as a brown powder

Visualization of stained cells was achieved with tyramide-enhanced FITC or rhodamine (1:50 dilution in amplification solution

FIGURE 3 3.3 Nitazoxanide reduced inflammation in rats with MTX-hepatotoxicity A profound hepatic inflammation was observed in the MTX group, as demonstrated by significant augmentation in hepatic TNF-, IL-1, and IL-6 versus the CTRL group (Figures 4AC)