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3 H-hypoxanthine uptake assays were performed by adding 3 H-hypoxanthine (American Radiolabeled Chemicals, Inc., ART 0266) (5 Ci per ml culture volume) to the cultures

Zhu J, Zheng X, Lu D, Zheng Y, Liu J

Interestingly, their effects on GH synthesis seem to be concentration-dependent, as low and high concentrations of nesfatins downregulate the expression of GH mRNA, while medium concentrations of nesfatins does not produce this effect (443)

As shown in the simulation, whether using only the dynamical system without medication (4) or with medication (2), it is not possible to entirely cure the patient (since the M2 density is increasing)