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Western blot 4T1 tumor tissues or HUVECs from treated and untreated groups were lysed with RIPA buffer containing protease and phosphatase inhibitor

Shotgun metagenomics analysis of faecal samples To explore the microbial metabolic function and ARGs of the rat faecal microbiota, DNA extracts of the representative samples were further subjected to shotgun metagenomics sequencing analysis on an Illumina HiSeq 4000 platform using the HiSeq 4000 PE Cluster Kit and HiSeq 4000 SBS Kits

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