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Abbreviations ALSFRS-R: Revised amyotrophic lateral sclerosis functional rating scale CSF: Cerebrospinal fluid Cys: Cysteine GSH: Reduced glutathione GSH*: Glycine- 13 C 2 , 15 N-Glutathione tGSH: Total glutathione GS-NEM: Derivatisation of GSH by NEM tGS-NEM: Derivatisation of tGSH by NEM GSSG: Oxidised glutathione HC: Healthy control group HPLC: High-pressure liquid chromatography ALS: Amyotrophic lateral sclerosis MRM: Multiple reaction monitoring MS: Mass spectrometry NEM: N-Ethylmaleimide TCEP: Tris(2-carboxyethyl)phosphine References Barber SC, Mead RJ, Shaw PJ

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The incidence of diarrhea, presented as a percentage, was calculated according to the following equation: Calves with a score of 2, received 4 L of daily oral electrolytes, divided into two meals, two hours after offering a milk replacer and a lactobacilli-based probiotic, until the consistency of the feces reduced the score