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Name: killer klows froms outer Klowns from Space From the sci How Did Killer Klowns From
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Western blotting Cell pellets were lysed for 30 min in a lysis buffer (50 Mm Tris HCl pH 7.6, 150 mM NaCl, and 1% (v/v) NP-40) containing complete protease inhibitor cocktail (Sigma-Aldrich, 4693159001)

Free heme has also been shown to promote inflammatory gene transcription in macrophages via TLR4 and NLRP3 inflammasome signaling, which have both been implicated in worsened atherosclerosis 72,73,74,75 (Fig