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548 Under conditions of normal oxygen levels (normoxia), the activity of HIF-1 is suppressed via redox-dependent modifications

It should be of note for this step that introduction of two equally overlapped deletions to a target segment of a selected BAC-reporter construct is a key to minimize experimental efforts the most: two equally overlapped deletions could narrow the critical region for transcriptional regulations into 1/3rd of the target segment (Fig