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Considering the low yield of expressed protein with pAcF incorporated, we engineered pCNFRS to improve the incorporation of pAcF by introducing a quadruple mutation H283T/P284S/M285D/D286V (TSDV) located at the anticodon recognition domain that was proved useful in pAzFRS for improving the incorporation of (p-azido-phenylalanine) pAzF 28

These features allow them to: (i) passively diffuse between corneocytes (skin cells) due to their small size, (ii) enhance permeation through controlled modifications of surface properties, and (iii) target specific receptors on skin cells using ligands, ultimately leading to deeper delivery of the encapsulated drug

However, some require supplementation

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