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After TMB substrate solution is added, only those wells that contain Human BMG/b2-MG, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color

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doi: 10.3390/nu9040367 Summary Keywords extrapineal melatonin, circadian rhythms, suprachiasmatic nucleus, mitochondria, redox homeostasis, free radicals, cerebrospinal fluid, cell metabolism Citation Reiter RJ, Sharma R, Tan D-X, Chuffa LGA, da Silva DGH, Slominski AT, Steinbrink K and Kleszczynski K (2024) Dual sources of melatonin and evidence for different primary functions

It acts as a mediator that converts these harmful elements into water-soluble forms and thus enables them to be excreted via the kidneys