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For neonatal medications, preservative-free sterile water must be used instead

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As a result, the pharmacodynamic profiles of these compounds include resting periods, when GLP-1 receptors are not activated, and periods of several hours when effective medication concentrations are present in the circulation

The reaction mixture, comprising 100 l 3 mM Gly-Phe-pNA substrate, 100 l appropriately diluted enzyme solution, 500 l 100 mM Tris-HCl buffer (pH 8.0) and 300 l water, was incubated at 303 K for 120 min