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Description

Using a reverse transcriptase enzyme mix and a PCR thermocycler, the first strand of cDNA was generated from 1 to 2 g of total RNA

Biondi B, Cappola AR, Cooper DS

Antibodies specific for the mouse cell surface markers CD11b, Ly6C, Ly6G, CD45, CD140a (PDGFRa), ANNEXIN V and F4/80 were used and are listed in Supplementary Table 1